No, getting a Pap smear will not affect your period. Two tools are used to take a Pap sample, one called a spatula, the other a cytobrush. This second tool looks like a mascara brush and is used to scrape cells from your cervix. Sometimes mild bleeding or spotting can occur with this scraping, but this is not your period. Having your period can interfere with the Pap sample, so assure you are not menstruating the day of your appointment. Easier said than done, I know. Too often seems that our period will show up just when the appointment we have been waiting for does!